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KMID : 0903520050480040411
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2005 Volume.48 No. 4 p.411 ~ p.417
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Modulatory Effects of 21 kinds of Medicinal Herbs Including Herba Pogostemi (Agastache rugosa) on Nitric Oxide Production in Macrophage Cell line RAW 264.7 cells
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Kim Seung-Hyun
Kang Mi-Young
Nam Seok-Hyun
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Abstract
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Aqueous extracts were prepared from 21 medicinal herbs including Herba Pogostemi (Agastache rugosa) to examine their modulatory effects on NO production in mouse macrophage cell line RAW264.7 cells. While almost all medicinal herb extracts failed to show marked scavenging activities to NO produced by LPS stimulation, only Herba Pogostemi showed a rather strong induction of NO production in RAW264.7 cells without stimulation with LPS. When we treated the cell with 200 μM of NG-monomethyl-L-arginine (NGMMA), a NOS2 inhibitor, a significant reduction in NO production could be observed. Moreover, a treatment of 100 μM pyrrolidine dithiocarbamate (PDTC) led to about a 79% reduction of NO production. These results demonstrated that the aqueous extract of Herba Pogostemi might provide a second signal for the expression of NOS2 in RAW264.7 cells, and suggested that Herba Pogostemi induces NO production through L-argininedependent pathway.
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KEYWORD
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Herba Pogostemi, Agastache rugosa, Nitric oxide, NG-monomethyl-L-arginine, Pyrrolidine dithiocarbamate
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